A: In most situations, this problem can be solved by adding 100 – 150 μL of MQ/deionized water to the gel surface and waiting for 10 – 20 mins. If the problem still exists, we suggest that the diffusive and binding gels are eluted together. Vgel (mL) here is close to the volume of water used for rehydration provided that all water is absorbed by the gels. M (nmol or ng) is the mass of analyte accumulated in the binding layer. X is the dilution time. fe is the elution factor. Ve (mL) is the volume of eluent. Cicp (nmol/mL or ng/mL) is the concentration measured by ICP-MS or other relevant instruments.
